Lack of chromosome territoriality in yeast: Promiscuous rejoining of broken chromosome ends
- 26 November 1996
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 93 (24) , 13949-13954
- https://doi.org/10.1073/pnas.93.24.13949
Abstract
Various studies suggest that eukarytoic chromosomes may occupy distinct territories within the nucleus and that chromosomes are tethered to a nuclear matrix. These constraints might limit interchromosomal interactions. We have used a molecular genetic test to investigate whether the chromosomes of Saccharomyces cerevisiae exhibit such territoriality. A chromosomal double-strand break (DSB) can be efficiently repaired by recombination between flanking homologous repeated sequences. We have constructed a strain in which DSBs are delivered simultaneously to both chromosome III and chromosome V by induction of the HO endonuclease. The arrangement of partially duplicated HIS4 and URA3 sequences around each HO recognition site allows the repair of the two DSBs in two alternative ways: (i) the creation of two intrachromosomal deletions or (ii) the formation of a pair of reciprocal translocations. We show that reciprocal translocations are formed approximately as often as the pair of intrachromosomal deletions. Similar results were obtained when one of the target regions was moved from chromosome V to any of three different locations on chromosome XI. These results argue that the broken ends of mitotic chromosomes are free to search the entire genome for appropriate partners; thus, mitotic chromosomes are not functionally confined to isolated domains of the nucleus, at least when chromosomes are broken.Keywords
This publication has 41 references indexed in Scilit:
- Scaffold-associated regions: cis-acting determinants of chromatin structural loops and functional domainsPublished by Elsevier ,2005
- In vivo biochemistry: Physical monitoring of recombination induced by site‐specific endonucleasesBioEssays, 1995
- Chromosome condensation and sister chromatid pairing in budding yeast.The Journal of cell biology, 1994
- SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeresCell, 1993
- The onset of homologous chromosome pairing during Drosophila melanogaster embryogenesis.The Journal of cell biology, 1993
- Role of Chromosome Territories in the Functional Compartmentalization of the Cell NucleusCold Spring Harbor Symposia on Quantitative Biology, 1993
- DNA loops: structural and functional properties of scaffold‐attached regionsMolecular Microbiology, 1992
- Double-strand breaks stimulate alternative mechanisms of recombination repairJournal of Molecular Biology, 1989
- Chromosomal ARS and CEN elements bind specifically to the yeast nuclear scaffoldCell, 1988
- Directionality and Regulation of Cassette Substitution in YeastCold Spring Harbor Symposia on Quantitative Biology, 1984