SELECTIVE GROWTH OF RABBIT CORNEAL EPITHELIAL-CELLS IN CULTURE AND BASEMENT-MEMBRANE COLLAGEN-SYNTHESIS
- 1 January 1980
- journal article
- research article
- Vol. 19 (10) , 1222-1230
Abstract
Selective isolation of rabbit corneal epithelial cells was achieved with a medium containing D-valine. Long-term cultures of the epithelial cells synthesize basement membrane-type procollagen doublet chains of 160,000 and 180,000 daltons and a higher MW species of 280,000 daltons. Some of these components are disulfide-linked. These peptides gave rise to a single pepsin-resistant collagen chain of about 115,000 daltons.This publication has 10 references indexed in Scilit:
- Production and characterization of a monoclonal antibody to chicken type I collagen.Proceedings of the National Academy of Sciences, 1979
- Characterization of a type IV procollagen synthesized by human amniotic fluid cells in culture.Journal of Biological Chemistry, 1979
- The localization and synthesis of some collagen types in developing mouse embryosCell, 1979
- Characterization of Pepsin Fragments of Basement Membrane Collagen Obtained from a Mouse TumorEuropean Journal of Biochemistry, 1979
- Collagen synthesis by cultures of stromal cells from normal human and keratoconus corneasBiochemical and Biophysical Research Communications, 1979
- PARTIAL CHARACTERIZATION OF HUMAN COLLAGEN AND PROCOLLAGEN SECRETED BY HUMAN CORNEAL STROMAL FIBROBLASTS IN CELL-CULTURE1978
- Heterogeneity of Pepsin-Solubilized Human Glomerular Basement Membrane CollagenNephron, 1978
- Cultured epithelial cells of cornea, conjunctiva and skin: absence of marked intrinsic divergence of their differentiated statesNature, 1977
- Evidence for heterogeneous origin of glomerular basement membraneBiochemical and Biophysical Research Communications, 1977
- The covalent structure of cartilage collagen. Evidence for sequence heterogeneity of bovine alpha1(II) chains.Journal of Biological Chemistry, 1977