Tissue‐specific induction of sister chromatid exchanges by ethyl carbamate in mice
- 1 January 1980
- journal article
- research article
- Published by Wiley in Environmental Mutagenesis
- Vol. 2 (1) , 17-26
- https://doi.org/10.1002/em.2860020104
Abstract
Sister chromatid exchange (SCE) techniques were used to analyze the genetic effects of ethyl carbamate (urethane) in cultured mouse-bone marrow cells, and in several different mouse tissues in vivo. Ethyl carbamate concentrations up to 5.0 mg/ml were ineffective in causing a significant elevation of SCE in vitro. After in vivo drug administration, bone marrow, liver and spermatogonial cells all revealed significant dose-related increases in SCE. Baseline and relative incremental levels of SCE were somatic vs germ tissue-specific. Regenerating liver cells exhibited significantly greater absolute SCE values than all other tissues examined. Marrow cells revealed intermediate values, while germ cells were the least sensitive in SCE responsiveness. Spermatogonia required a fourfold higher dose, over that effective in somatic tissues, to promote an approximate doubling of the baseline SCE level. In vivo SCE analysis affords sensitive risk assessments for different tissues. Thus, this approach should be generally useful for studying compounds with questionable mutagenic potential, and/or those exerting target organ specificities of related biological activity (eg, toxicity, carcinogenesis).Keywords
This publication has 44 references indexed in Scilit:
- Sister chromatid exchange in regenerating liver and bone marrow cells of mice exposed to styreneToxicology and Applied Pharmacology, 1979
- Spontaneous and mitomycin-C induced sister-chromatid exchangesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979
- In vivo sister-chromatid exchange: A sensitive measure of DNA damageMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979
- Factors influencing the frequency of mitomycin C-induced sister-chromatid exchanges in 5-bromodeoxyuridine-substituted human lymphocytes in cultureMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
- Analysis of sister chromatid exchange formation in vivo in mouse spermatogonia as a new test system for environmental mutagensNature, 1976
- Differential chromatid staining by in vivo treatment as a mutagenicity test systemNature, 1976
- Sister chromatid exchanges induced in chinese hamster cells by UV irradiation of different stages of the cell cycle: The necessity for cells to pass through SMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
- Mutagenic response of known carcinogens : Kennedy Jr., G. L., D. W. Arnold and M. L. Keplinger, Industrial BIO-TEST Labs., Inc., Northbrook, Ill. (U.S.A.)Mutation Research/Environmental Mutagenesis and Related Subjects, 1973
- Latent Meiotic Anomalies Related to an Ancestral Exposure to a Mutagenic AgentScience, 1971
- A failure to detect any mutagenic action of urethane in the mouseMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1967