Effects of peroxynitrite‐induced protein modifications on tyrosine phosphorylation and degradation
Open Access
- 29 April 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 385 (1-2) , 63-66
- https://doi.org/10.1016/0014-5793(96)00347-x
Abstract
The ability of protein tyrosine kinases to phosphorylate a synthetic peptide was inhibited 51% by peroxynitritemediated nitration of tyrosine. Exposure of endothelial cells to peroxynitrite decreased the intensity of tyrosine phosphorylated proteins and increased the intensity of nitrotyrosine‐containing proteins. Peroxynitrite‐modified BSA was degraded by human red blood cell lysates. However, human plasma in a concentration‐, time‐, and temperature‐dependent manner, removed the protein nitrotyrosine epitope. These results suggest that tyrosine nitration interferes with phosphorylation and targets proteins for degradation. Specific enzymatic process(es) for removing nitrotyrosine may be present in vivo.Keywords
This publication has 21 references indexed in Scilit:
- Peroxynitrite‐Mediated Inhibition of DOPA Synthesis in PC12 CellsJournal of Neurochemistry, 1995
- Peroxynitrite‐mediated oxidative protein modificationsFEBS Letters, 1995
- Endotoxin triggers the expression of an inducible isoform of nitric oxide synthase and the formation of peroxynitrite in the rat aorta in vivoFEBS Letters, 1995
- INHIBITION OF NEURONAL NITRIC-OXIDE SYNTHASE BY 7-NITROINDAZOLE PROTECTS AGAINST MPTP-INDUCED NEUROTOXICITY IN MICEJournal of Neurochemistry, 1995
- Quantitation of nitrotyrosine levels in lung sections of patients and animals with acute lung injury.Journal of Clinical Investigation, 1994
- Evidence for nitric oxide‐mediated oxidative damage in chronic inflammation Nitrotyrosine in serum and synovial fluid from rheumatoid patientsFEBS Letters, 1994
- Peroxynitrite‐mediated oxidation of albumin to the protein‐thiyl free radicalFEBS Letters, 1994
- Aromatic hydroxylation and nitration of phenylalanine and tyrosine by peroxynitriteFEBS Letters, 1994
- Inactivation of .alpha.1-proteinase inhibitor by peroxynitriteChemical Research in Toxicology, 1992
- Stimulation of tyrosine-specific protein phosphorylation in the rat liver plasma membrane by oxygen radicalsBiochemical and Biophysical Research Communications, 1986