Cyclic AMP-dependent modification of gonad-selective TAFII105 in a human ovarian granulosa cell line

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Abstract
In response to gonadotropins, the elevated level of intracellular‐cyclic AMP (cAMP) in ovarian granulosa cells triggers an ordered activation of multiple ovarian genes, which in turn promotes various ovarian functions including folliculogenesis and steroidogenesis. Identification and characterization of transcription factors that control ovarian gene expression are pivotal to the understanding of the molecular basis of the tissue‐specific gene regulation programs. The recent discovery of the mouse TATA binding protein (TBP)‐associated factor 105 (TAFII105) as a gonad‐selective transcriptional co‐activator strongly suggests that general transcription factors such as TFIID may play a key role in regulating tissue‐specific gene expression. Here we show that the human TAFII105 protein is preferentially expressed in ovarian granulosa cells. We also identified a novel TAFII105 mRNA isoform that results from alternative exon inclusion and is predicted to encode a dominant negative mutant of TAFII105. Following stimulation by the adenylyl cyclase activator forskolin, TAFII105 in granulosa cells undergoes rapid and transient phosphorylation that is dependent upon protein kinase A (PKA). Thus, our work suggests that pre‐mRNA processing and post‐translational modification represent two important regulatory steps for the gonad‐specific functions of human TAFII105.