Biochemical Basis of Resistance to Hygromycin B in Streptomyces hygroscopicus - the Producing Organism
- 1 June 1985
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 131 (6) , 1289-1298
- https://doi.org/10.1099/00221287-131-6-1289
Abstract
Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by S. hygroscopicus. Ribosomes from this gram-negative mycelial bacterium are inhibited in vitro by the antibiotic. The streptomycete is highly resistant to the drug in vivo since it possesses hygromycin B phosphotransferase activity. This enzyme was shown by gel filtration to have a MW of 42,000. It modifies its antibiotic substrate to produce 7''''-O-phosphoryl-hygromycin B, which totally lacks biological activity in vivo and in vitro.This publication has 15 references indexed in Scilit:
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