Translation of virus mRNA: synthesis of bacteriophage Q beta proteins in a cell-free extract from wheat embryo.
- 1 December 1973
- journal article
- research article
- Vol. 12 (6) , 1434-41
Abstract
The RNA from bacteriophage Qbeta can be translated by cell-free extracts from wheat embryos. This translation, by 80S ribosomes, occurs at a low magnesium ion concentration. Three products are synthesized which coelectrophorese with Qbeta proteins synthesized in Escherichia coli extracts. The smallest of these has been identified as coat protein. Although the polycistronic bacteriophage message is translated with fidelity, the efficiency is much less than when the monocistronic brome mosaic virus coat protein message is translated.This publication has 21 references indexed in Scilit:
- Efficient synthesis of rabbit globin in a cell‐free system from wheat embryoFEBS Letters, 1973
- Translation of bacteriophage R17 and Qβ RNA in a mammalian cell-free systemJournal of Molecular Biology, 1973
- Translation of Bacteriophage Qβ Messenger RNA in a Murine Krebs 2 Ascites Tumor Cell-Free SystemScience, 1972
- Translation of satellite tobacco necrosis virus ribonucleic acid. I. Characterization of in vitro procaryotic and eucaryotic translation productsBiochemistry, 1972
- Origin of the small component of brome mosaic virus RNAJournal of Molecular Biology, 1972
- Translation of in vitro synthesized reovirus messenger RNAs into proteins of the size of reovirus capsid proteins in a mouse L cell extractBiochemical and Biophysical Research Communications, 1972
- Ribosomal proteins of Escherichia coli. II. Chemical and physical characterization of the 30 S ribosomal proteinsBiochemistry, 1969
- Cell-free protein synthesis programmed with R17 RNA: Identification of two phage proteinsJournal of Molecular Biology, 1966
- Purification and properties of bacteriophage MS2 and of its ribonucleic acidJournal of Molecular Biology, 1963
- Mass Isolation of Viable Wheat EmbryosNature, 1957