Inhibition of classical PKC isoenzymes downregulates STAT1 activation and iNOS expression in LPS‐treated murine J774 macrophages

Open Access
Publisher Website
Google Scholar
Abstract
Proinflammatory cytokines and bacterial products trigger inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production in inflammatory and tissue cells. In inflammation, NO acts as an important mediator having both proinflammatory and destructive effects. Protein kinase C (PKC) is a family of serine–threonine protein kinase isoenzymes involved in signal transduction pathways related to inflammatory responses. The aim of the present study was to investigate the role of classical PKC (cPKC) isoenzymes in the regulation of iNOS expression and NO production in murine J774 macrophages and the mechanisms involved. RO318220 (inhibits PKCβ, PKCγ and PKCɛ), GÖ6976 (inhibits cPKC isoenzymes PKCα and PKCβ) and LY333531 (inhibits PKCβ) reduced lipopolysaccharide (LPS)‐induced NO production and iNOS expression in a dose‐dependent manner as did 6 h pretreatment with 1 μM phorbol 12‐myristate 13‐acetate (PMA) (which was shown to downregulate PKC expression). PKC inhibitors also reduced LPS‐induced iNOS mRNA levels, but they did not affect the half‐life of iNOS mRNA. PKC inhibitors did not alter LPS‐induced activation of NF‐κB as measured by electrophoretic mobility shift assay. All PKC inhibitors used and pretreatment with 1 μM PMA inhibited signal transducer and activator of transcription 1 (STAT1) activation as measured by the translocation of STAT1α from the cytosol to the nucleus by Western blot. In addition, inhibition of STAT1 activation by AG‐490, an inhibitor of JAK‐2, also reduced NO production. These results suggest that cPKC isoenzymes, especially PKCβ, mediate the upregulation of iNOS expression and NO production in activated macrophages in an NF‐κB‐independent manner, possibly through the activation of transcription factor STAT1. British Journal of Pharmacology (2006) 147, 790–799. doi:10.1038/sj.bjp.0706672

This publication has 50 references indexed in Scilit: