Immunoassay Using a Metal-complex Compound as a Chemiluminescent Catalyst. III. Flow-through Analysis of a Labeled Antigen Bound by Immune Reaction

Abstract
By measuring the chemiluminescence intensity by the flow-through method rather than the conventional batchwise method, the sensitivity and precision of immunoassay and the time required for the measurement of the chemiluminescence intensity were improved as follows: 20 ng–50 μg of human albumin per tube, 5.8–7.2% (n=9) coefficient of variation, and 4 min measuring time.