Presence of nitric oxide synthase activity in roots and nodules of Lupinus albus
- 2 December 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 398 (2-3) , 159-164
- https://doi.org/10.1016/s0014-5793(96)01232-x
Abstract
NO is a widespread messenger molecule in physiology. We were interested in investigating whether an NO-generating system could be present in plants. NO and l-[14C]citrulline were synthesized by roots and nodules of Lupinus albus in an l-arginine-dependent manner. l-[14C]Citrulline production was inhibited by NG-monomethyl-l-arginine, a nitric oxide synthase antagonist, in a competitive way. NADPH-diaphorase activity was localized in the vascular bundles in root and nodules, and also in the nodule infected zone. This staining was significantly reduced in the presence of NG-monomethyl-l-arginine. These results indicate the presence of a putative nitric oxide synthase in plants.Keywords
This publication has 26 references indexed in Scilit:
- Localization of Lipoxygenase Proteins and mRNA in Pea Nodules: Identification of Lipoxygenase in the Lumen of Infection ThreadsMolecular Plant-Microbe Interactions®, 1996
- L-Arginine transport and metabolism in Giardia intestinalis support its position as a transition between the prokaryotic and eukaryotic kingdomsMicrobiology, 1995
- Localization of nitric oxide synthase in the adult rat brainPhilosophical Transactions Of The Royal Society B-Biological Sciences, 1994
- Heme-Based Sensors, Exemplified by the Kinase FixL, Are a New Class of Heme Protein with Distinctive Ligand Binding and AutoxidationBiochemistry, 1994
- Cytological Evidence for a Host Defense Response That Reduces Cell and Tissue Invasion in Pea Nodules by Lipopolysaccharide-Defective Mutants ofRhizobium leguminosarumStrain 3841Molecular Plant-Microbe Interactions®, 1994
- Comparison of Substrate and Inhibitor Specificity of Arginase and Nitricm Oxide (NO) Synthase for Arginine Analogs and Related Compounds in Murine and Rat MacrophagesBiochemical and Biophysical Research Communications, 1994
- Nitric oxide synthase activity from a hematophagous insect salivary glandFEBS Letters, 1993
- Phorbol esters induce nitric oxide synthase and increase arginine influx in cultured peritoneal macrophagesFEBS Letters, 1993
- Nodule growth and activity may be regulated by a feedback mechanism involving phloem nitrogen*Plant, Cell & Environment, 1993
- Mammalian Nitric Oxide SynthasesPublished by Wiley ,1992