Phorbol esters induce nitric oxide synthase and increase arginine influx in cultured peritoneal macrophages
- 5 April 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 320 (2) , 135-139
- https://doi.org/10.1016/0014-5793(93)80078-9
Abstract
Incubation of peritoneal macrophages with β‐phorbol 12,13‐dibutyrate promotes a time‐dependent release of NO to the incubation medium. This effect was antagonized by LPS, a well known inducer of nitric oxide synthase (NOS) expression in macrophages, and was inhibited by N G‐methyl‐l‐arginine and N ω‐nitro‐l‐arginine. An increase in intracellular cGMP and NOS activity was observed in parallel with NO release. The induction of NOS was accompanied by a stimulation of arginine influx within the cell. These results suggest that activation of protein kinase C by phorbol esters is sufficient to promote NOS induction in macrophages.Keywords
This publication has 24 references indexed in Scilit:
- The role of protein kinase C in the induction of nitric oxide synthesis by murine macrophagesBiochemical and Biophysical Research Communications, 1992
- Interleukin-8 inhibits the induction of nitric oxide synthase in rat peritoneal neutrophilsBiochemical and Biophysical Research Communications, 1992
- Lipopolysaccharide and cytokines induce a macrophage-type of nitric oxide synthase in bovine retinal pigmented epithelial cellsBiochemical and Biophysical Research Communications, 1992
- Mechanistic probes of N-hydroxylation of L-arginine by the inducible nitric oxide synthase from murine macrophagesBiochemistry, 1992
- Substrate‐dependent inhibition of protein kinase C by specific inhibitorsFEBS Letters, 1990
- Novel human brain cDNA encoding a 34,000 Mr protein n-chimaerin, related to both the regulatory domain of protein kinase C and BCR, the product of the breakpoint cluster region geneJournal of Molecular Biology, 1990
- THE PROTEIN KINASE C FAMILY: HETEROGENEITY AND ITS IMPLICATIONSAnnual Review of Biochemistry, 1989
- Activation of protein kinase C from B lymphocytes by lipid ABiochemical and Biophysical Research Communications, 1988
- Analysis of nitrate, nitrite, and [15N]nitrate in biological fluidsAnalytical Biochemistry, 1982
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976