Expression of human alpha 1-antitrypsin cDNA in the yeast Saccharomyces cerevisiae.

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Abstract
Nucleotide sequences coding either for the precursor or the mature form of human .alpha.1-antitrypsin were placed under the control of the yeast ARG3 expression signals [to produce large quantities of .alpha.-antitrypsin to assess its therapeutic value relative to emphysema]. Recombinant plasmids pRIT10782 and pRIT10787 express the precursor or the mature .alpha.1-antitrypsin species, respectively, in 2 different yeast strains, with yields ranging between 0.3-1% of total soluble proteins. The .alpha.1-antitrypsin synthesized in yeasts was specifically recognized by polyclonal and monoclonal antibodies raised against human .alpha.1-antitrypsin. It was biologically active in its mature form only, with optimal activity in a peptidase-deficient yeast strain.