Metabolic Inhibition Test for Determination of Antibodies to Group B Coxsackie Viruses.

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Abstract
Serodiagnosis of infections due to Group B Coxsackie viruses is made practical by a modification of the Salk polio metabolic inhibition test. This test employs Eagles KB (human carcinoma of the nasopharynx) cells; five replicate serial dilutions of acute and convalescent sera, each of which receives a Group B Coxsackie virus diluted to contain 102.0 to 103-0 ID50 (infectious doses); and appropriate controls. The test is read after incubation at 37[degree]C for approximately one week, red color denoting failure of the cells to metabolize and a yellow color indicating growth of the cells. A 4-fold or greater rise in antibody titer is indicative of infection with Group B Coxsackie virus. This test permits serodiagnosis in 80-90% of cases where the acute phase serum is drawn within 4 days of the onset of illness. However, the infecting type is not determined unless there is a monotypic antibody rise or the study is accompanied by a positive virus isolation. Persons possessing neutralizing antibodies from previous Group B virus infections frequently show heterologous as well as homologous antibody increases.