Purification and spectroscopic characterization of a recombinant amino‐terminal polypeptide fragment of mouse epithelial cadherin
- 3 October 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 352 (3) , 318-322
- https://doi.org/10.1016/0014-5793(94)00982-1
Abstract
Cadherins are a family of Ca2+-dependent cell adhesion molecules containing four extracellular tandem repeats each of 110 amino acids. The most amino-terminal repeat is believed to confer the specificity of cell adhesion. A polypeptide containing the amino-terminal repeat of mouse epithelial cadherin has been over-expressed in E. coli and purified to homogeneity. This polypeptide binds Ca2+ with a dissociation constant of 1.6 × 10−4M. CD and NMR experiments indicate that the polypeptide adopts a predominantly β-sheet conformation and that binding of Ca2+ induces only small conformational changes.Keywords
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