Coactivator and Promoter-Selective Properties of RNA Polymerase I TAFs
- 1 December 1995
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 270 (5241) , 1506-1509
- https://doi.org/10.1126/science.270.5241.1506
Abstract
Human ribosomal RNA synthesis by RNA polymerase I requires the activator UBF and the promoter selectivity factor SL1, which consists of the TATA binding protein (TBP) and three associated subunits, TAFI110, TAFI63, and TAFI48. Here it is shown that both TAFI110 and TAFI63 contact the promoter, whereas TAFI48 serves as a target for interaction with UBF and is required to form a transcriptionally active SL1 complex responsive to UBF in vitro. TAFI48 also alters the ability of TBP to interact with TATA box elements, and the resulting complex fails to support transcription by RNA polymerase II. Thus, TAFI48 may function both as a target to mediate UBF activation and as a class-specific promoter selectivity factor.Keywords
This publication has 24 references indexed in Scilit:
- Assembly of Transcriptionally Active RNA Polymerase I Initiation Factor SL1 from Recombinant SubunitsScience, 1994
- Reconstitution of Transcription Factor SL1: Exclusive Binding of TBP by SL1 or TFIID SubunitsScience, 1994
- Multiple regions of TBP participate in the response to transcriptional activators in vivo.Genes & Development, 1994
- Assembly of recombinant TFIID reveals differential coactivator requirements for distinct transcriptional activatorsCell, 1994
- TBP-TAF complexes: selectivity factors for eukaryotic transcriptionCurrent Opinion in Cell Biology, 1994
- Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex.Genes & Development, 1993
- TBP, a universal eukaryotic transcription factor?Genes & Development, 1993
- Multiple functional domains of human transcription factor IIB: distinct interactions with two general transcription factors and RNA polymerase II.Genes & Development, 1993
- The TATA-binding protein and associated factors are integral components of the RNA polymerase I transcription factor, SL1Cell, 1992
- Human rRNA transcription is modulated by the coordinate binding of two factors to an upstream control elementCell, 1986