In vitro translation of the citrus tristeza virus coat protein from a 0.8 kbp double-stranded RNA segment

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Abstract
Several dsRNA segments, ranging in size from 0.8 to 19.5 kbp, have been isolated from citrus plants infected with the VT and ST strains of citrus tristeza virus (CTV) and translated, after denaturation, in a reticulocyte lysate cell-free system. Only two small dsRNA segments of 0.8 and 1.7 kbp were efficiently translated and showed a consistent pattern of products. The major translation product of the 0.8 kpb dsRNA was a 27K polypeptide, immunoprecipitated by antiserum to the CTV coat protein. The major translation product of the 1.7 kbp dsRNA segment was a 21.5K peptide. The possibility that the CTV coat protein may be translated from a subgenomic viral messenger RNA is discussed.