Purification of Equine Gonadotropin and Its Effect on the Appearance of Antigonadotropic Substances in Human Sera12

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Abstract
Patients suffering from amenorrhea were treated with either of 2 preps. of equine gonadotrophin derived from pregnant mare serum, one being 2.5 times more highly purified than the other on the basis of their N content. Six of 7 patients injected with the less purified prep. developed antigonado-trophic substances to equine gonadotrophin in their blood. This was usually 1st evident in 9-10 wks. after the initial inj. and persisted for at least 3 mos. after cessation of treatment, thus rendering equine gonadotrophin therapy essentially valueless. However, with the more highly purified prep. only 1 of 12 patients developed antigonadotrophic serum. These results indicate that increased purification of equine gonadotrophin will markedly lower the tendency for the formation of antigonadotrophins in the human. The sera of 39 amenorrheic [female][female] tested prior to hormone therapy show that antigonadotrophins of equine gonadotrophin are normally absent.

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