MORPHOLOGICAL, BIOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF HUMAN BLADDER TRANSITIONAL CELL CARCINOMAS GROWN IN TISSUE-CULTURE AND IN NUDE-MICE

  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (9) , 3997-4005
Abstract
The morphological, biological, biochemical and karyotypic characteristics of 4 human bladder transitional cell carcinoma lines, SW-780, SW-800, SW-1738 and SW-1710, were investigated. In tissue culture, each cell line presented a distinct phenotypic expression. All but line SW-1710 grew when transplanted in the nude mouse. Light microscopic and EM studies showed morphological characteristics similar to the tumors of origin, being independent of the passages in tissue culture or the nude mouse. Lactate dehydrogenase was present in culture medium, tumor cell extracts and the plasma of nude mouse-grown tumors, showing isoenzyme quantitative distribution typical for each cell line. Each cell line exhibited a unique genetically determined enzyme phenotypic profile which, along with the karyotypic analysis, makes their identification feasible. These characteristics make the described tumor lines a valuable tool in studying various aspects of the biology of human bladder transitional cell carcinoma.