Chemically modified nucleic acids as immunodetectable probes in hybridization experiments.
- 1 June 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (11) , 3466-3470
- https://doi.org/10.1073/pnas.81.11.3466
Abstract
Guanine residues in nucleic acids can be modified by treatment with N-acetoxy-N-2-acetylaminofluorene and its 7-iodo derivative in an in vitro nonenzymatic reaction. The modified nucleic acids (ribo or deoxyribo, single or double stranded) are recognized by specific antibodies. They can be immunoprecipitated or used as probes in hybridization experiments and detected by immunochemical techniques.This publication has 28 references indexed in Scilit:
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