Enhancement of Oncogenesis in C3H/10T1/2 Mouse Embryo Cell Cultures by Saccharin

22 September 1978

journal article
research article
Published by American Association for the Advancement of Science (AAAS) in Science

Vol. 201 (4361) , 1141-1142
https://doi.org/10.1126/science.684434

Abstract

Impure and pure samples of saccharin (2 milligrams per milliliter) did not produce oncogenic transformation of C3H/10T1/2, clone 8, mouse embryo fibroblasts. However, after treatment of the cells with a nontransforming initiating dose (0.1 microgram per milliliter) of 3-methylcholanthrene, continuous treatment with either sample of saccharin (100 micrograms per milliliter) led to significant transformation. It is concluded that in this system saccharin is a cocarginogen, probably functioning as a promoting agent that is 1000-fold less active than the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate.

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