2-STAGE CHEMICAL ONCOGENESIS IN CULTURES OF C3H-10T1-2 CELLS

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (7) , 2254-2260
Abstract
The initiation and promotion stages of chemical oncogenesis were demonstrated in cultured C3H/10T1/2 mouse embryo cells. Treatment of the cells with a subeffective concentration of 3-methylcholanthrene, benzo(a)pyrene or 7,12-dimethylbenz[a]anthracene, followed 4 days later by a nontransforming amount of tetradecanoylphorbol acetate (TPA), phorbol didecanoate or 4-.alpha.-phorbol didecanoate, produced transformation. Phorbol was ineffective. TPA did not select for transformed cells. When TPA treatment preceded 3-methylcholanthrene, no enhancement of transformation was observed. When TPA was added immediately after hydrocarbon treatment, there was a significant inhibition of transformation. TPA did not exert promoting activity when the hydrocarbons were in high enough concentrations to produce appreciable transformation. The promoting action of TPA cannot be attributed only to a stimulation of cell division.

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