Polarized absorption picosecond kinetics as a probe of energy transfer in phycobilisomes ofSynechococcus6301

Abstract
The energy transfer between C‐phycocyanin chromophores in intact phycobilisomes ofSynechococcus6301 is shown to lead to an anisotropy relaxation with a lifetime of 10 ± 2 ps. However, due to the molecular order within the hexameric units of C‐phycocyanin the anisotropy does not decay to zero. The Förster dipole‐dipole mechanism of energy transfer can qualitatively explain these data provided that there is no back transfer of excitation energy and that the chromophore distribution is non‐random. The rate of energy transfer in phycobilisomes between C‐phycocyanin and allophycocyanin can best be described by a double exponential with lifetimes of 12 ± 3 and 84 ± 8 ps.

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