The Isolation of Deoxyribonucleic Acid from Virulent and Avirulent Strains of Haemophilus pertussis
- 1 May 1951
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 5 (2) , 268-275
- https://doi.org/10.1099/00221287-5-2-268
Abstract
Deoxyribonucleic acid may be extracted from H. pertussis by 2% Na cholate by a method outlined. The deoxyribonucleic acid is isolated in a comparatively undegraded form free from ribonucleic acid and protein, but containing a polysaccharide. Some properties of the deoxyribonucleic acids of a virulent and an avirulent strain of H. pertussis are compared. It is suggested that the greater difficulties experienced in extracting deoxyribonucleic acid from virulent cells might be due to a firm linkage with the polysaccharide, possibly at the cell surface.Keywords
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