Upstream sequences confer distinctive transcriptional properties on genes encoding silkgland-specific tRNAAla.
- 1 January 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (2) , 374-378
- https://doi.org/10.1073/pnas.83.2.374
Abstract
To understand the molecular basis for the tissue-specific accumulation of alanine tRNAs in silkworms, we have compared the transcriptional properties of genes that encode constitutive and silkgland-specific type alanine tRNA. Genes for each class of alanine tRNA behave very differently when used as templates for homologous in vitro transcription. Since these properties are likely related to the activities of the corresponding genes in vivo, we wished to identify the cis-acting elements responsible for them. We have therefore constructed hybrid silkgland-specific/constitutive genes and have analyzed their capacity to direct transcription in vitro. We find a simple pattern: the distinctive transcriptional properties of the two kinds of tRNAAla genes are the result of different positive signals upstream from their sites of transcription initiation.This publication has 14 references indexed in Scilit:
- A large region controls tRNA gene transcriptionJournal of Molecular Biology, 1985
- Steady‐state kinetic analysis of transcription of cloned tRNASer genes from Drosophila melanogasterEuropean Journal of Biochemistry, 1985
- In vitro transcription of a silkworm 5S RNA gene requires an upstream signal.Proceedings of the National Academy of Sciences, 1984
- A short 5′ flanking region containing conserved sequences is required for silkworm alanine tRNA gene activityProceedings of the National Academy of Sciences, 1983
- A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragmentsGene, 1982
- 5′ flanking sequence signals are required for activity of silkworm alanine tRNA genes in homologous in vitro transcription systemsCell, 1980
- The primary transcription product of a silkworm alanine tRNA gene: Identification of in vitro sites of initiation, termination and processingCell, 1979
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- The nucleotide sequence of two silk gland alanine tRNAs: Implications for fibroin synthesis and for initiator tRNA structureCell, 1977
- Specific alanine‐tRNA species associated with fibroin biosynthesis in the posterior silk‐gland of Bombyx mori L.FEBS Letters, 1977