Transcriptional (p40x) and post-transcriptional (p27x-III) regulators are required for the expression and replication of human T-cell leukemia virus type I genes.
- 1 June 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (11) , 3653-3657
- https://doi.org/10.1073/pnas.84.11.3653
Abstract
The pX sequence of human T-cell leukemia virus type I codes for three products: p40x, p27x-III, and p21x-III. p40x is a transcriptional trans-activator that activates not only the viral long terminal repeat but also cellular genes for interleukin 2 and its receptor. p27x-III and p21x-III are not require for transcriptional activation, and their functions were unknown. Cotransfection experiments with defective human T-cell leukemia virus type I proviruses and various pX expression plasmids revealed that p27x-III, in addition to p40x, was required for gag gene expression. Furthermore, it was shown that p27x-III induced accumulation of a high level of unspliced viral gag mRNA. These results indicate that p27x-III is a post-transcriptional modulator of viral RNA whose transcription has been fully activated by p40x.This publication has 38 references indexed in Scilit:
- Identification of p26xb and p24xb of human T‐cell leukemia virus type IIFEBS Letters, 1986
- The second pX product p27x‐III of HTLV‐1 is required for gag gene expressionFEBS Letters, 1986
- Requirement of multiple copies of a 21-nucleotide sequence in the U3 regions of human T-cell leukemia virus type I and type II long terminal repeats for trans-acting activation of transcription.Proceedings of the National Academy of Sciences, 1986
- Cis-acting sequences responsible for the transcriptional activation of human T-cell leukemia virus type I constitute a conditional enhancer.Proceedings of the National Academy of Sciences, 1986
- HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNACell, 1986
- A second post-transcriptional trans-activator gene required for HTLV-III replicationNature, 1986
- Nucleotide sequence of the protease-coding region in an infectious DNA of simian retrovirus (STLV) of the HTLV-I familyVirology, 1986
- THE P40X OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I IS A TRANS-ACTING ACTIVATOR OF VIRAL GENE-TRANSCRIPTION1985
- The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase geneNucleic Acids Research, 1980
- Inhibition of intractable, nucleases with ribonucleoside-vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytesBiochemistry, 1979