THE P40X OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I IS A TRANS-ACTING ACTIVATOR OF VIRAL GENE-TRANSCRIPTION
- 1 December 1985
- journal article
- research article
- Vol. 76 (12) , 1127-1131
Abstract
Human T-cell leukemia virus type I has a unique sequence pX and the product p40x was proposed to be a specific trans-acting transcriptional activator of expression of the viral gene. Recently, a second pX protein p27x-111 in addition to p40x was identified; these two proteins are encoded by overlapping frames III and IV (x-lor). For determination of which product is the trans-acting activator, site-directed mutations were introduced into the pX sequence which was placed under the metallothionein promoter. On cotransfection with pLTR-CAT (a plasmid containing the LTR or HTLV-I and chloramphenicol acetyltransferase gene), only the mutations that affected p40x expression inactivated the transcriptional activation from the LTR.This publication has 14 references indexed in Scilit:
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