POTENTIATION OF LYMPHOCYTE MITOGENIC RESPONSES TO CONCANAVALIN-A BY ANTIGEN-ACTIVATED PERIPHERAL-BLOOD MONOCYTES

  • 1 January 1979
    • journal article
    • research article
    • Vol. 40  (5) , 684-687
Abstract
Purified protein derivative (PPD)-stimulated monocytes derived from Mycobacterium bovis-sensitized cattle significantly potentiated lymphocyte mitogenic responses to concanavalin (Con)A, as measured by incorporation of [3H] thymidine into cellular DNA. Monocytes were cultured for 24 h in the presence of PPD, washed thoroughly, and mixed with purified lymphocytes. Various doses of ConA were added to these cultures, and the the cultures were incubated for 4 days and assayed for DNA synthesis. The lymphocyte mitogenic responses to suboptimal, but not optimal, doses of ConA were significantly enhanced by the presence of PPD-activated monocytes from M. bovis-sensitized cattle. Treatment of monocytes or lymphocytes (or both) from normal nonsensitized cattle with PPD did not result in any significant enhancement of ConA-induced lymphocyte mitogenic responses at any dose of ConA tested.