Decreased peptidyltransferase activity correlates with increased programmed −1 ribosomal frameshifting and viral maintenance defects in the yeast Saccharomyces cerevisiae
Open Access
- 17 July 2003
- journal article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 9 (8) , 982-992
- https://doi.org/10.1261/rna.2165803
Abstract
Increased efficiencies of programmed −1 ribosomal frameshifting in yeast cells expressing mutant forms of ribosomal protein L3 are unable to maintain the dsRNA “Killer” virus. Here we demonstrate that changes in frameshifting and virus maintenance in these mutants correlates with decreased peptidyltransferase activities. The mutants did not affect Ty1-directed programmed +1 ribosomal frameshifting or nonsense-mediated mRNA decay. Independent experiments demonstrate similar programmed −1 ribosomal frameshifting specific defects in cells lacking ribosomal protein L41, which has previously been shown to result in peptidyltransferase defects in yeast. These findings are consistent with the hypothesis that decreased peptidyltransferase activity should result in longer ribosome pause times after the accommodation step of the elongation cycle, allowing more time for ribosomal slippage at programmed −1 ribosomal frameshift signals.Keywords
This publication has 65 references indexed in Scilit:
- The Genome Sequence of the SARS-Associated CoronavirusScience, 2003
- Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory SyndromeScience, 2003
- An ‘integrated model’ of programmed ribosomal frameshiftingTrends in Biochemical Sciences, 2002
- Ribosomal Pausing at a Frameshifter RNA Pseudoknot Is Sensitive to Reading Phase but Shows Little Correlation with Frameshift EfficiencyMolecular and Cellular Biology, 2001
- Expression of a Micro-proteinJournal of Biological Chemistry, 2001
- The Complete Atomic Structure of the Large Ribosomal Subunit at 2.4 Å ResolutionScience, 2000
- A comparison of the yeast and rabbit 80 S ribosome reveals the topology of the nascent chain exit tunnel, inter-subunit bridges and mammalian rRNA expansion segmentsJournal of Molecular Biology, 2000
- The 3D arrangement of the 23 S and 5 S rRNA in the Escherichia coli 50 S ribosomal subunit based on a cryo-electron microscopic reconstruction at 7.5 Å resolutionJournal of Molecular Biology, 2000
- Cold‐Sensitive Ribosome Assembly in an Esclzerichia coli Mutant Lacking a Single Methyl Group in Ribosomal Protein L3European Journal of Biochemistry, 1981
- Evidence of the involvement of a 50S ribosomal protein in several active sitesBiochemistry, 1975