Transport mechanism and metabolism of olive oil hydroxytyrosol in Caco‐2 cells
- 27 March 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 470 (3) , 341-344
- https://doi.org/10.1016/s0014-5793(00)01350-8
Abstract
3,4-dihydroxyphenylethanol (hydroxytyrosol; DPE) is the major phenolic antioxidant present in extra virgin olive oil, either in a free or esterified form. Despite its relevant biological effects, no data are available on its bioavailability and metabolism. The aim of the present study is to examine the molecular mechanism of DPE intestinal transport, using differentiated Caco-2 cell monolayers as the model system. The kinetic data demonstrate that [(14)C]DPE transport occurs via a passive diffusion mechanism and is bidirectional; the calculated apparent permeability coefficient indicates that the molecule is quantitatively absorbed at the intestinal level. The only labelled DPE metabolite detectable in the culture medium by HPLC (10% conversion) is 3-hydroxy-4-methoxyphenylethanol, the product of catechol-O-methyltransferase; when DPE is assayed in vitro with the purified enzyme a K(m) value of 40 microM has been calculated.Keywords
This publication has 29 references indexed in Scilit:
- Inhibition of peroxynitrite dependent DNA base modification and tyrosine nitration by the extra virgin olive oil-derived antioxidant hydroxytyrosolFree Radical Biology & Medicine, 1999
- Determination of Synthetic Hydroxytyrosol in Rat Plasma by GC-MSJournal of Agricultural and Food Chemistry, 1998
- Free Radical-Scavenging Properties of Olive Oil PolyphenolsBiochemical and Biophysical Research Communications, 1998
- 'In vitro' evaluation of the antioxidant activity and biomembrane interaction of the plant phenols oleuropein and hydroxytyrosolInternational Journal of Pharmaceutics, 1998
- Kinetics of Human Soluble and Membrane-Bound Catechol O-Methyltransferase: A Revised Mechanism and Description of the Thermolabile Variant of the EnzymeBiochemistry, 1995
- Dietary fat, olive oil intake and breast cancer riskInternational Journal of Cancer, 1994
- Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly StudyThe Lancet, 1993
- Simple and hydrolyzable phenolic compounds in virgin olive oil. 1. Their extraction, separation, and quantitative and semiquantitative evaluation by HPLCJournal of Agricultural and Food Chemistry, 1992
- Correlation between oral drug absorption in humans and apparent drug permeability coefficients in human intestinal epithelial (Caco-2) cellsBiochemical and Biophysical Research Communications, 1991
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976