Detection of a novel minisatellite-specific DNA-binding protein
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (3) , 625-629
- https://doi.org/10.1093/nar/18.3.625
Abstract
We describe the detection of a ubiquitous DNA-binding protein which appears to interact specifically with tandem-repeated minisatellites. The murine 40kd protein, which we term Msbp-1, was found to be present in all mouse tissues tested. This protein was bound specifically and with high affinity by double-stranded DNA containing a repeat sequence related to the minisatellite ''core'' sequence, and binding required the presence of multiple repeat units. Corresponding minisatellite-specific DNA-binding protein could also be detected in species ranging from Drosophila to man. This analysis represents the first direct evidence that minisatellites can function as a specific recognition signal for an endogenous DNA-binding protein.This publication has 27 references indexed in Scilit:
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