Die Aminosäuresequenz des doppelköpfigen Proteinaseninhibitors aus der Glandula submandibularis des Hundes, II. Ein Methioninrest als reaktives Hemmzentrum für Chymotrypsin

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Abstract
The canine submandibular inhibitor is double-headed with 2 independent reactive sites. Whereas the trypsin-reactive center (-Ala-Cys-Pro-Arg26-Leu-His-) is located in domain I, the chymotrypsin-reactive site (-Met-Cys-Thr-Met78-Asp-Tyr-) is located in domain II. The presence of a methionine residue in this inhibition center is supported by the findings that nitration with tetranitromethane abolishes neither trypsin nor chymotrypsin inhibition; after alkylation of the methione residues, only trypsin inhibition is retained. Another inhibitor from microbial sources which also contains a methionine residue in the presumed reactive site also inhibits subtilisin but not chymotrypsin (or trypsin).

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