Indium (111In)-Labelled Human Platelets: Optimal Method

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Abstract
A detailed laboratory method is described for the labeling of human platelets with [111In]indium oxine. The 45 min method is simple, requires only 26 ml of blood and is suitable for routine clinical use. After the labeling and resuspension of the platelets in plasma, aggregation responses to both ADP and collagen were similar to those of normal platelet-rich plasma. Less than 5% of the [111In] indium oxine was released by secretory functions of platelets. Labeling efficiencies of 90.1 .+-. 4.29% (n = 28) were achieved in 60 s by normal concentrations of plasma-free platelet suspensions. Platelet survival in vivo in healthy volunteer subjects follows a linear function with a survival time of 8.44 .+-. 0.18 days.