Nucleohistone as a primer for RNA synthesis.

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Abstract
Native calf thymus nucleohistone (DNP) is a poor primer for RNA synthesis in vitro. Sonicated calf thymus DNP, however, has one third the priming efficiency of equivalent amounts of calf thymus DNA, when the primer concentration is 20 [mu]g/ml of DNA or lower. In the incubation mixture, native DNP forms an impenetrable gel-like precipitate, whereas sonicated DNP forms a dispersed precipitate. DNA does not precipitate in the incubation mixture. The in vitro reduction, by histone, of DNA-primed RNA synthesis is due to this precipitation effect, rather than to a genetic repression of the regulator-operator type.