Enhanced Accuracy and Reliability of HER-2/neu Immunohistochemical Scoring Using Digital Microscopy

1 May 2004

journal article
research article
Published by Oxford University Press (OUP) in American Journal of Clinical Pathology

Vol. 121 (5) , 620-630
https://doi.org/10.1309/y73u-8x72-b68t-mgh5

Abstract

We evaluated the HER-2/neu status of 129 invasive breast cancer specimens for gene amplification by fluorescence in situ hybridization (FISH) and protein overexpression by immunohistochemical analysis. Each immunohistochemically stained slide was interpreted on a standard microscope independently by 10 pathologists. Separately, each pathologist reviewed the same slide set with the assistance of digital microscopy.

Keywords

This publication has 27 references indexed in Scilit:

Assessment of HER-2/neu Status in Breast Cancer
American Journal of Clinical Pathology, 2001
Use of Chemotherapy plus a Monoclonal Antibody against HER2 for Metastatic Breast Cancer That Overexpresses HER2
New England Journal of Medicine, 2001
Chromogenic in Situ Hybridization
The American Journal of Pathology, 2000
HER-2/neu Testing in Breast Carcinoma: A Combined Immunohistochemical and Fluorescence In Situ Hybridization Approach
Laboratory Investigation, 2000
HER-2/neu Gene Amplification Compared With HER-2/neu Protein Overexpression and Interobserver Reproducibility in Invasive Breast Carcinoma
American Journal of Clinical Pathology, 2000
Loss of Tumor Marker-Immunostaining Intensity on Stored Paraffin Slides of Breast Cancer
JNCI Journal of the National Cancer Institute, 1996
C-erbB-2 immunostaining: problems with interpretation.
Journal of Clinical Pathology, 1994
Humanization of an anti-p185HER2 antibody for human cancer therapy.
Proceedings of the National Academy of Sciences, 1992
Human Breast Cancer: Correlation of Relapse and Survival with Amplification of the HER-2/ neu Oncogene
Science, 1987