Enhanced fMLP-stimulated chemotaxis in human neutrophils from individuals carrying the G protein β3 subunit 825 T-allele
- 2 October 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 436 (2) , 155-158
- https://doi.org/10.1016/s0014-5793(98)01110-7
Abstract
We have recently described a C825T polymorphism in the gene encoding for the Gβ3 subunit of heterotrimeric G proteins. The 825T allele is associated with a novel splice variant (Gβ3-s) and enhanced signal transduction via pertussis toxin (PTX)-sensitive G proteins. fMLP-induced chemotaxis, but not O2− generation, was increased in neutrophils with the TC/TT (EC50=1.5±1.3 nM) genotypes compared to the CC genotype (EC50=5.9±1.5 nM). Maximal fMLP-induced increase in [Ca2+]i was significantly reduced in neutrophils from individuals with TC/TT genotype vs. CC genotype (212.9±10.1 nM vs. 146.4±24.2 nM). Gβ3-s appears to be associated with enhanced immune cell function in humans.Keywords
This publication has 16 references indexed in Scilit:
- Association of a human G-protein β3 subunit variant with hypertensionNature Genetics, 1998
- Pertussis Toxin-sensitive Activation of Phospholipase C by the C5a and fMet-Leu-Phe ReceptorsJournal of Biological Chemistry, 1996
- A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13.Genome Research, 1996
- Enhanced G protein activation in immortalized lymphoblasts from patients with essential hypertension.Journal of Clinical Investigation, 1995
- The role of G-proteins in the activation of human leukocytes by particulate stimuli to produce reactive oxygen metabolitesToxicology, 1995
- Subunit expression of signal transducing G proteins in cardiac tissue: Implications for phospholipase C-β regulationJournal of Molecular and Cellular Cardiology, 1995
- Cloning and sequencing of a rat heart cDNA encoding a G-protein β subunit related to the human retinal β3 subunitGene, 1994
- The active role of βγ in signal transductionCurrent Opinion in Cell Biology, 1994
- Signal transduction in N‐formyl‐methionyl‐leucyl‐phenylalanine and concanavalin A stimulated human neutrophils: Superoxide production without a rise in intracellular free calciumJournal of Cellular Physiology, 1990
- The Glomerular Permeability Determined by Dextran Clearance Using Sephadex Gel FiltrationScandinavian Journal of Clinical and Laboratory Investigation, 1968