Synthesis of a photoaffinity-spin-labeled derivative of ATP and its first application to F1-ATPase
- 25 January 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 227 (2) , 107-109
- https://doi.org/10.1016/0014-5793(88)80878-0
Abstract
The synthesis of an ATP derivative is described, in which a spin-label is attached to the 3′-position of the ribose moiety and an azido group to C8 of the adenine ring (SL-N3-ATP). Irradiation of this compound at 350 nm generates a nitrene, which will react with any functional group in its vicinity. SL-N3-ATP exhibits a strongly immobilized ESR spectrum in a complex with F1-ATPase from beef heart mitochondria. It was covalently incorporated into this enzyme. SL-N3-ATP may be employed in ESR investigations under conditions in which non-covalent interactions are too weak for motionally restricted species to be easily observed.Keywords
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