Expression and integration of a foreign gene in orange (Citrus sinensis Osb.) protoplasts by direct DNA transfer.
- 1 January 1989
- journal article
- research article
- Published by Genetics Society of Japan in The Japanese Journal of Genetics
- Vol. 64 (2) , 91-97
- https://doi.org/10.1266/jjg.64.91
Abstract
Protoplasts isolated from suspension cultured cells or orange (Citrus sinensis Osb.) were treated with a bacterial plasmid DNA carrying a chimeric gene consisting of the nopaline synthase promoter, the aminoglycoside phosphotransferase II [APH(3'')II] structural gene from the bacterial transposon Tn 5 and a terminator region from cauliflower mosiac virus DNA. Colonies capable of proliferating in a medium containing kanamycin (25 .mu.g/ml) possessed APH(3'')II activity and an intact foreign gene. The transformation frequency was recorded to be in the order of 10-6.This publication has 16 references indexed in Scilit:
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