INACTIVATION OF ULTRAVIOLET REPAIR IN NORMAL AND XERODERMA PIGMENTOSUM-CELLS BY METHYL METHANESULFONATE
- 1 January 1982
- journal article
- research article
- Vol. 42 (3) , 860-863
Abstract
Excision repair of UV damage in the DNA of normal and xeroderma pigmentosum (groups C, D and variant) cells [XP7CA, XP8BE, XP1AA, XP1BR and XP115LO cell lines] was inactivated by exposure of cells to methyl methanesulfonate immediately before irradiation independent of the presence of 0-10% fetal calf serum. The inactivation could be represented by a semilog relationship between the amount of repair and methyl methanesulfonate concentration up to .apprx. 5 mM. The inactivation can be considered to occur as the result of alkylation of a large (about 106 daltons) repair enzyme complex, and the dose required to reduce repair to 37% for most cell types was between 4-7 mM. No consistent, large difference in sensitivity to methyl methanesulfonate was found in any xeroderma pigmentosum complementation group compared to normal cells, implying that reduced repair in these groups may be caused by small inherited changes in the amino acid composition (i.e., point mutations or small deletions) rather than by losses of major components of the repair enzyme complex.This publication has 14 references indexed in Scilit:
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