Two restriction endonucleases from Bacillus globiggi
- 1 July 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (7) , 1747-1760
- https://doi.org/10.1093/nar/3.7.1747
Abstract
The sites of action of the restriction enzyme Bgl II on lambda DNA are mapped. This enzyme recognises the sequence 5' ...AGATCT...3' and makes staggered cuts producing sticky ends. In lambda DNA, the second A in this sequence is methylated about 50% of the time by a bacterial methylase absent in E. coli dam. In contrast to Bgl II, Bgl I makes many cuts in lambda DNA and produces 5' terminals which are not substrates for polynucleotide kinase.Keywords
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