Collagen Films: Effect of Cross-Linking on Physical and Biological Properties

Abstract
Enzyme-solubilized collagen was cast into films with different fibrillar structures and cross-linked with glutaraldehyde. Cross-linked films had decreased swelling ratios, decreased degree of hydrolysis, increased resistance to bacterial collagenase, and increased shrinkage temperatures. Subcutaneous implantation in rabbits indicated that cross-linked films could survive for at least 90 days virtually unchanged and surrounded by a fibrous cuff. Cells were able to grow on the collagen surfaces. These studies indicate that collagen, a natural polymer, can be used where long-lasting biomaterials are required.