Immunohistochemical Localization of ?-Mannosidase During Postnatal Development of the Rat Cerebellum

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Abstract
The localizaton of .alpha.-D-mannosidase in the rat cerebellum was studied by using indirect immunohistochemistry at both optical microscopic and EM levels. In the adult the enzyme is particularly concentrated in the dendrites and cell bodies of Purkinje cells, basket cells and Golgi neurons in the cerebellar cortex and in the cytoplasm and dendrites of deep nuclei neurons. The cytoplasm of granule cells is poorly stained; parallel fibers, white matter, Bergman fibers and Golgi epitheloid cell perikarya show virtually no staining. EM suggests that most of the staining is found in the cytosol, although some staining is found in the postsynaptic densities of the synapses between parallel fibers and Purkinje dendrites. The pattern of staining was followed throughout the postnatal development of the rat cerebellum. At birth an intense and diffuse staining is found in all cells except those of the external germinative layer. At the 6th postnatal day, Purkinje cell bodies and apical cones are strongly labeled. From the 13th day on, the pattern is very similar to that found in the adult. At the 18th postnatal day (when compared with the other structures), the staining of Purkinje cell dendrites seems to be higher than that at all other ages. These data are correlated with biochemical studies and discussed in relation to the possible role of this enzyme during the postnatal development of the rat cerebellum.