Specificity Analysis of Antibodies Formed in Rabbits to a Mannosyl Trisaccharide: Similarity with Lectin Binding Activity Para-Aminophenyl O-α-D-Mannopyranosyl-(1→2)-α-D-Mannopyranosyl-(1→6)-α-D-Mannopyranoside Linked to Bovine Serum Albumin as an Antigen

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Abstract
The para-aminophenyl derivative of Manα1→2Manα1→6Manα1→ was coupled via a diazotization reaction to bovine serum albumin, and the resulting glycoconjugate was used to immunize two rabbits. The resultant antisera were tested for reactivity with a number of related mono, di- and trisaccharides to determine the immunodominant portion of this trisaccharide. Two populations of antibody resulted, one of which required the reducing end mannose, and could react with either an N-acetylglucosamine or a mannose as the penultimate sugar. The other population reacted with the Manα1→2Manα1→6Manα1→. The aglycone moiety and its configurations play an important role in determining the specificity of antibodies to this synthetic antigen. The similarity of this reactivity to the reactivity of mannose binding lectins is discussed.

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