Synthesis of low molecular weight RNA components A, C and D by polymerase II in α-amanitin-resistant hamster cells
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (1) , 321-330
- https://doi.org/10.1093/nar/6.1.321
Abstract
In an attempt to establish which RNA polymerase catalyzes the synthesis of the low molecular weight RNA components A, C and D, Ama 1 cells (mutant Chinese hamster cells) were used in experiments with addition of alpha-amanitin. Ama 1 cells contain an altered RNA polymerase II which is 800 times more resistant towards inhibition by alpha-amanitin than the wild type enzyme. Alpha-amanitin (up to 200 microgram/ml) added to these cells does not affect the synthesis of the low molecular weight RNAs A, C and D. These data together with our previous data showing that alpha-amanitin (0.5 - 5.0 microgram/ml) preferentially inhibits the synthesis of A, C and D in normal cells indicate that RNA polymerase II catalyzes the synthesis of the low molecular weight RNA components A, C and D.Keywords
This publication has 24 references indexed in Scilit:
- Inhibition of nucleoplasmic transcription and the translation of rapidly labeled nuclear proteins by low concentrations of actinomycin D in vivo. Proposed role of messenger RNA in ribosomal RNA transcriptionBiochemistry, 1978
- The differential inhibitory effect of α‐amanitin on the synthesis of low molecular weight RNA components in BHK cellsFEBS Letters, 1978
- Synthesis of two classes of small RNA species in vivo and in vitroBiochemistry, 1977
- Transcription of specific genes in isolated nuclei by exogenous RNA polymerasesCell, 1977
- The presence of small molecular weight RNAs in nuclear ribonucleoprotein particles carrying HnRNAFEBS Letters, 1977
- RNA polymerase B from an α-amanitin resistant mouse myeloma cell lineFEBS Letters, 1976
- The 7S RNA Common to Oncornaviruses and Normal Cells is Associated with PolyribosomesProceedings of the National Academy of Sciences, 1974
- Mammalian Cells with Altered Forms of RNA Polymerase IIProceedings of the National Academy of Sciences, 1972
- Two Types of Ribosome in Mouse–Hamster Hybrid CellsNature New Biology, 1971
- Metabolism of small molecular weight monodisperse nuclear RNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969