Manganese superoxide dismutase content and localization in human thyroid tumours

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Abstract
Manganese‐containing superoxide dismutase (Mn‐SOD) content and its immunohistochemical localization in human thyroid tumours and some other thyroid diseases were examined and compared with adjacent normal thyroid tissue. Enzyme‐linked immunosorbent assay (ELISA) was used in this study for the measurement of Mn‐SOD. The content of Mn‐SOD tended to increase in diffuse hyperplasia, adenomatous goitre, and foliicular adenoma. In papillary carcinoma, it was significantly higher than in adjacent normal thyroid tissue. Foliicular carcinoma also revealed a markedly high Mn‐SOD conient. In the immunohistochemicai study, adjaceni normal thyroid tissue showed granular positive staining of Mn‐SOD in the cytoplasm. An increase of Mn‐SOD was observed in the papillary proliferative lesion of diffuse hyperplasia and in the follicles adjacent to lymphoid tissue in chronic thyroiditis with hypothyroidism. Strong positive staining of Mn‐SOD was observed in papillary and foliicular carcinomas, whereas in anaplastic carcinoma staining was markedly less intense. These results indicate that the Mn‐SOD content varies according to the degree of differentiation of thyroid carcinomas.