AB (TYPE V) AND BASEMENT MEMBRANE (TYPE IV) COLLAGENS IN THE BOVINE LUNG PARENCHYMA: ELECTRON MICROSCOPIC LOCALIZATION BY THE PEROXIDASE-LABELED ANTIBODY METHOD

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Abstract
The microdistribution of AB collagen and basement membrane collagen in the bovine lung parenchyma was studied by the immunoelectron microscopic method. AB collagen was isolated from the pepsin digests of bovine lung parenchyma and purified with differential salt fractionation. Identification and purity of the collagen were assessed by SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis, collagenase digestion, phosphocellulose chromatography, amino acid analysis and electrophoresis of CNBr-cleavage products. Type specific rabbit antibodies to AB and type IV collagens showing no cross-reaction with other types of collagen were prepared by the immunoadsorption technique. Immunoelectron microscopic studies using the indirect peroxidase-labeled antibody method showed that AB collagen is located on the surfaces of varied cells, such as bronchiolar and alveolar epithelial cells, vascular endothelial cells and alveolar septal cells. Type IV collagen is located along basement membranes of alveoli and capillaries.