Tricalcium phosphate as an adsorbent in the chromatography of proteins

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Abstract
Hydrated tricalcium phosphate as an adsorbent for proteins was investigated with respect to the effects of phosphate buffer, pH and NaCl on the chromatography of phycoerythrin. Phosphate buffer is an effective displacing agent. In low concns. it gives elution analysis of phycoerythrin with considerable tailing, and in higher concns. it gives displacement analysis in sharp bands. Variations in pH have little effect. NaCl in low concns. promotes to a great extent the adsorption of phycoerythrin, and to a lesser extent the adsorption of phosphate buffer.

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