Some observations on the form and location of invertase in the yeast cell

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Abstract
Saccharomyces cerevisiae, S. carlsbergensis when treated with ethyl acetate and thoroughly washed with water retain the greater part of their invertase, but S. fragilis loses it to the suspending medium. When ethyl-acetate-treated cells were disrupted in the Hughes (1951) press almost the whole of their content of invertase was liberated in a soluble form. The enzyme was also wholly liberated by the action of crop juice of Helix pomatia and partly liberated by the action of papain preparations. Snail-crop juice also liberated the greater part of the invertase from living yeast cells, even under conditions in which the protoplasts remained intact The action of both enzyme preparations in liberating invertase from living or ethyl-acetate-treated cells was enhanced by the presence of cysteine. It is concluded that the invertase of the yeast cell occurs in a soluble form in a region of the cell outside the protoplast but inside the cell wall, and not, as previously supposed, in combination with insoluble cell structures.