Surfection: a new platform for transfected cell arrays
Open Access
- 13 February 2004
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 32 (3) , 33e-33
- https://doi.org/10.1093/nar/gnh029
Abstract
Efficient high‐throughput expression of genes in mammalian cells can facilitate large‐scale functional genomic studies. Towards this aim, we developed a simple yet powerful method to deliver genes into cells by cationic polymers on the surface of substrates. Transfection can be achieved by directly contacting nucleic acid–cell mixtures with the cationic substrates, e.g. polyethylenimine/collagen‐coated wells. This single‐step matrix‐surface‐ mediated transfection method, termed ‘surfection’, can efficiently deliver multiple plasmids into cells and can successfully assay siRNA‐mediated gene silencing. This technology represents the easiest method to transfer combinations of genes in large‐scale arrays, and is a versatile tool for live‐cell imaging and cell‐based drug screening.Keywords
This publication has 34 references indexed in Scilit:
- RNAi Microarray Analysis in Cultured Mammalian CellsGenome Research, 2003
- Atelocollagen-Based Gene Transfer in Cells Allows High-Throughput Screening of Gene FunctionsBiochemical and Biophysical Research Communications, 2001
- Microarrays go live – new prospects for proteomicsTrends in Biochemical Sciences, 2001
- Collapsin Response Mediator Protein-1 and the Invasion and Metastasis of Cancer CellsJNCI Journal of the National Cancer Institute, 2001
- NoBP, a Nuclear Fibroblast Growth Factor 3 Binding Protein, Is Cell Cycle Regulated and Promotes Cell GrowthMolecular and Cellular Biology, 2001
- High Throughput Screening Method for Identification of New Lipofection ReagentsSLAS Discovery, 2001
- Microarrays of cells expressing defined cDNAsNature, 2001
- Gene delivery from a DNA controlled-release stent in porcine coronary arteriesNature Biotechnology, 2000
- Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coralProceedings of the National Academy of Sciences, 2000
- Application of membrane-based dendrimer/DNA complexes for solid phase transfection in vitro and in vivoBiomaterials, 2000