Recovery and Repopulation in Vivo by Mouse Skin Epithelial Cells during Fractionated Irradiation

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Abstract
The dose survival and regeneration characteristics of irradiated mouse skin epithelial cells were studied using an in situ, in vivo cell cloning technique. The cells surviving a first dose of X-rays are characterized by 24 hr. later by a survival curve having the same slope as that for cells not previously irradiated. The value for (D2-D1)24hr. was about 350 to 375 rads. The value of (D2-D1) increased by an average of about 100 rads/day with extension of fractionation intervals from 1 to 6 days. At intervals of less than 24 hr., a typical fluctuating pattern of recovery was observed. It is postulated that some repopulation by surviving cells occurs within 24 hr. of a first dose of less than 800 rads and that this proceeds in the 5 days thereafter with a doubling time for the clonogenic population of approximately 22 hr. Evidence is presented for the differentiation of about 25% of each generation of cells during rapid re-epithelialization.

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