A factor binding GATAAG confers tissue specificity on the promoter of the humanζ-globin gene
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (6) , 1339-1350
- https://doi.org/10.1093/nar/18.6.1339
Abstract
We describe the characterization of cis-acting sequences which control the tissue specific expression of the human .zeta. globin gene. An extensive search for enhancer sequences in the vicinity of this gene proved negative. Instead our data demonstrate that the minimal promoter of the .zeta. gene is itself tissue specific. Sequences close to and possibly including the - 100 CACCC and - 70 CCAAT boxes display some erythroid specificity. However the principal tissue specific element is a GATAA sequence at -120 directly adjacent to the minimal promoter. Specific deletion of GATAA reduces .zeta. promoter activity 5-fold in erythroid but not non-erythroid cells. We also demonstrate that an erythroid specific factor binds to this GATAA sequence. Furthermore this factor forms a complex with the transcription factor CP1 which we show interacts with the .zeta. CCAAT box. We present evidence that the .zeta. GATAA binding factor is equivalent to GF1 recently purified and cloned by Tsai et al. The erythroid specific GATAA sequence has been found in the promoters and enhancers of a number of erythroid specific genes. Similarly we show here that the .zeta. globin gene relies on a GATAA sequence in its promoter to specify its expression in erythroid cells.This publication has 30 references indexed in Scilit:
- Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cellsNature, 1989
- Increased γ-globin expression in a nondeletion HPFH mediated by an erythroid-specif ic DNA-binding factorNature, 1989
- A yeast and a human CCAAT-binding protein have heterologous subunits that are functionally interchangeableCell, 1988
- Position-independent, high-level expression of the human β-globin gene in transgenic miceCell, 1987
- A cellular DNA-binding protein that activates eukaryotic transcription and DNA replicationCell, 1987
- Homologous recognition of a promoter domain common to the MSV LTR and the HSV tk geneCell, 1986
- Two distinct enhancers with different cell specificities coexist in the regulatory region of polyomaCell, 1984
- The structure of the human zeta-globin gene and a closely linked, nearly identical pseudogeneCell, 1982
- Erythroleukemic DifferentiationAnnual Review of Biochemistry, 1978
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973